NGS hybrid capture is a detection method that designs specific probes for the genomic region of interest, hybridizes it with genomic DNA, enriches the DNA fragments of the target genomic region, and then uses high-throughput sequencing technology for sequencing. The introduction of an automated liquid processing platform in this method can greatly increase the processing throughput and free researchers from repeated and complicated experimental work.
Automation solution
1. NGS hybrid capture flow chart
Remarks:
1. The part with orange background is a manual operation step. Because hybridization involves concentration and freeze-drying, automatic steps cannot be realized, so manual operations are used before and after the steps.
2. To prepare 1× hybridization eluent, manually prepare 1× hybridization eluent, and then pour it into the corresponding reagent tank of the workstation.
3. Automatically complete the hybridization capture process at the LH-1808 automatic liquid handling workstation
Step 1: Open the software and select the "hybrid capture" program
Step 2: Take 16 post-hybridization libraries as an example (up to 48 post-hybridization libraries), arrange the deck, as shown in the figure below
Step 3: Click "Start" to run, and the program will run.
4. Accessories and functions of LH-1808 automatic liquid handling workstation
The LH-1808 automatic liquid handling workstation helps users to realize the NGS hybridization and capture automation process, which not only ensures the repeatability and uniformity of the sample results, but also saves labor costs and shortens the detection time, which is very suitable for the development of high-throughput sequencing technology.